rat anti mouse il 22ra1  (R&D Systems)

Journal: Frontiers in Immunology

Article Title: IL-22-STAT3-CD155 axis in alveolar echinococcosis: a pivotal role in immune exhaustion and therapeutic potential

doi: 10.3389/fimmu.2025.1674904

Figure Lengend Snippet: IL-22 promotes CD155 expression through the IL-22RA1-STAT3 signaling axis. (A) Representative western blotting images of IL-22RA1, STAT3 and p-STAT3 expression in hepatocytes across experimental groups. (B) Statistical results of IL-22RA1, STAT3 and p-STAT3 expression in hepatocytes across experimental groups (n=3). (C) Immunohistochemical representative images of IL-22, IL-22RA1 and p-STAT3 in the liver tissues of mice at 1-month post-infection. Scale bar: 100μm. (D) Immunohistochemical representative images of IL-22, IL-22RA1 and p-STAT3 in the liver tissues of mice at 3-month post-infection. Scale bar: 100μm. (E) Statistical results of the positive area of IL-22, IL-22RA1 and p-STAT3 in the liver of mice at 1-month post-infection (n =5). (F) Statistical results of the positive area of IL-22, IL-22RA1 and p-STAT3 in the liver of mice at 3-month post-infection (n =5). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. ns, no significance.

Article Snippet: After blocking with 5% non-fat milk in TBST (pH 7.6) for 2 h at room temperature, membranes were incubated overnight at 4°C with the following primary antibodies: anti-IL-22RA1 rabbit polyclonal antibody (GB11302-100, 1:1000, Servicebio), anti-STAT3 rabbit polyclonal antibody (GB11176-100, 1:1000, Servicebio), and anti-CD155 rabbit polyclonal antibody (31447, 1:1000, Proteintech).

Techniques: Expressing, Western Blot, Immunohistochemical staining, Infection

Journal: Nature Communications

Article Title: Pancreatic beta-cell IL-22 receptor deficiency induces age-dependent dysregulation of insulin biosynthesis and systemic glucose homeostasis

doi: 10.1038/s41467-024-48320-2

Figure Lengend Snippet: a mRNA fold change of IL-22RA1 gene expression in healthy lean versus T2D human donor islets, relative to control GAPDH . b Total insulin, c proinsulin secretion (ng/10 islets/30 min), and d proinsulin: insulin ratio from human donor islets during glucose stimulated insulin secretion following treatment 10 µg mL −1 anti-IL22RA1 ( p = 0.0341 ). e mRNA fold change of spliced XBP-1 ( sXBP-1; p = 0.0225 ) and f NOS2 in human donor islets, relative to control GAPDH following treatment with 10 µg mL −1 anti-IL22RA1. g Intracellular nitrite production (µM) in human donor islets following 24 h treatment with 10 µg mL −1 anti-IL22RA1. All graphs presented as Mean ± SEM. a n = 3 biologically independent donors, Two-tailed Mann-Whitney Test; b–g n = 3 biologically independent human islet donors, Kruskal-Wallis test with Dunn’s multiple comparisons test. * p < 0.05; n.s., non-significant. *versus vehicle (anti-IgG) control. Source data are provided as a Source Data file.

Article Snippet: IL-22ra1-FLPe (B6.Cg- Il22ra1 tm1.1Koll /J; Jackson Laboratory Strain #:031003) animals were crossed with either Ins2-cre (B6.Cg-Tg(Ins2-cre)25Mgn/J; Jackson Laboratory Strain #:003573) or Gcg-cre (B6.Cg-Tg(Gcg-cre)1Herr/Mmnc/J; MMRCC, 000358-UNC) animals to generate Ins2 cre x IL-22ra1-FLPe and Gcg cre x IL-22ra1-FLPe colonies respectively.

Techniques: Gene Expression, Control, Two Tailed Test, MANN-WHITNEY

Journal: Nature Communications

Article Title: Pancreatic beta-cell IL-22 receptor deficiency induces age-dependent dysregulation of insulin biosynthesis and systemic glucose homeostasis

doi: 10.1038/s41467-024-48320-2

Figure Lengend Snippet: a Experimental schematic, created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license. b Pancreatic sections of β-cell IL-22ra1 knockout animals and their wildtype littermate counterparts stained for cre-recombinase, IL-22ra1 and Insulin. c Changes in glucose tolerance following oral glucose administration in animals with age. d Area under the curve during oral glucose tolerance tests in animals with age (16 weeks p = 0.0380 ; 20 weeks p = 0.0146 ). All graphs are presented as Mean ± SEM. Female animals; n = 7 biologically independent animals (8–16 weeks), 9 biologically independent animals (20 weeks), Two-tailed unpaired Student’s t-test. * p < 0.05; n.s., non-significant. *versus wildtype (IL-22ra fl/fl ) littermates. Scale Bar: 20 um. Source data are provided as a Source Data file.

Article Snippet: IL-22ra1-FLPe (B6.Cg- Il22ra1 tm1.1Koll /J; Jackson Laboratory Strain #:031003) animals were crossed with either Ins2-cre (B6.Cg-Tg(Ins2-cre)25Mgn/J; Jackson Laboratory Strain #:003573) or Gcg-cre (B6.Cg-Tg(Gcg-cre)1Herr/Mmnc/J; MMRCC, 000358-UNC) animals to generate Ins2 cre x IL-22ra1-FLPe and Gcg cre x IL-22ra1-FLPe colonies respectively.

Techniques: Knock-Out, Staining, Two Tailed Test

Journal: Nature Communications

Article Title: Pancreatic beta-cell IL-22 receptor deficiency induces age-dependent dysregulation of insulin biosynthesis and systemic glucose homeostasis

doi: 10.1038/s41467-024-48320-2

Figure Lengend Snippet: a Pancreatic sections stained for insulin and proinsulin. b Mean intensity per islet area of insulin and proinsulin. c Total serum insulin ( p = 0.0175 ) and proinsulin ( p = 0.0452 ) in animals at 20 weeks of age. d Serum proinsulin: insulin ratio ( e ) Heatmap showing mRNA fold change of insulin secretion and (f) insulin signaling markers, in whole pancreatic tissue, relative to control housekeeping gene Ywhaz . Box plots in ( b–d ) display the median (central line), 25 th to 75 th percentile (box) and minimum to maximum values (whiskers). Female animals; ( a – b ) n = 12 independent islets from 3 biologically independent animals (insulin), n = 15 independent islets from 3 biologically independent animals (WT, proinsulin), n = 12 independent islets from 3 biologically independent animals ( IL-22ra1 ΔβKO , proinsulin). c , d n = 8 biologically independent wildtype ( IL-22ra fl/fl ) and 9 biologically independent IL-22ra1 ΔβKO animals; e , f n = 7 biologically independent animals. Two-tailed unpaired Student’s t-test; * p < 0.05, ** p < 0.01; n.s., non-significant. *versus wildtype ( IL-22ra fl/fl ) littermates. Scale bar: 20 um. Source data are provided as a Source Data file.

Article Snippet: IL-22ra1-FLPe (B6.Cg- Il22ra1 tm1.1Koll /J; Jackson Laboratory Strain #:031003) animals were crossed with either Ins2-cre (B6.Cg-Tg(Ins2-cre)25Mgn/J; Jackson Laboratory Strain #:003573) or Gcg-cre (B6.Cg-Tg(Gcg-cre)1Herr/Mmnc/J; MMRCC, 000358-UNC) animals to generate Ins2 cre x IL-22ra1-FLPe and Gcg cre x IL-22ra1-FLPe colonies respectively.

Techniques: Staining, Control, Two Tailed Test

Journal: Nature Communications

Article Title: Pancreatic beta-cell IL-22 receptor deficiency induces age-dependent dysregulation of insulin biosynthesis and systemic glucose homeostasis

doi: 10.1038/s41467-024-48320-2

Figure Lengend Snippet: a H&E sections from pancreatic tissue. b Pancreatic islet area ( p < 0.0001 ), and c frequency distribution of islet areas by size from serial pancreatic sections in animals at 20 weeks of age. d Absolute islet counts in animals following pancreatic islet isolation at 20 weeks of age ( p < 0.0001 ). e Heatmap showing mRNA fold change of islet growth/regeneration markers in whole pancreatic tissue, relative to control housekeeping gene Ywhaz . f Immunohistochemical staining of pancreatic sections for Ki-67 at 8 weeks of age. g Number of Ki-67 + cells per islet area ( p = 0.0090 ). All graphs are presented as Mean ± SEM. Female animals; a – c n = 165 independent islets (all islets in serial sections) from 3 biologically independent wildtype ( IL-22ra fl/fl ), and 115 independent islets (all islets in serial sections) from 3 biologically independent IL-22ra1 ΔβKO animals. d n = 3 biologically independent wildtype ( IL-22ra fl/fl ) and 4 biologically independent IL-22ra1 ΔβKO animals. f , g n = 16 independent islets (all islets in one section) from 3 biologically independent wildtype ( IL-22ra fl/fl ), and 10 independent islets (all islets in one section) from 3 biologically independent IL-22ra1 ΔβKO animals. e n = 7 biologically independent animals; Two-tailed unpaired Student’s t-test. ** p < 0.01, **** p < 0.0001; n.s., non-significant. * versus wildtype (IL-22ra fl/fl ) littermates. Scale Bar: 50 um. Source data are provided as a Source Data file.

Article Snippet: IL-22ra1-FLPe (B6.Cg- Il22ra1 tm1.1Koll /J; Jackson Laboratory Strain #:031003) animals were crossed with either Ins2-cre (B6.Cg-Tg(Ins2-cre)25Mgn/J; Jackson Laboratory Strain #:003573) or Gcg-cre (B6.Cg-Tg(Gcg-cre)1Herr/Mmnc/J; MMRCC, 000358-UNC) animals to generate Ins2 cre x IL-22ra1-FLPe and Gcg cre x IL-22ra1-FLPe colonies respectively.

Techniques: Isolation, Control, Immunohistochemical staining, Staining, Two Tailed Test

Journal: Nature Communications

Article Title: Pancreatic beta-cell IL-22 receptor deficiency induces age-dependent dysregulation of insulin biosynthesis and systemic glucose homeostasis

doi: 10.1038/s41467-024-48320-2

Figure Lengend Snippet: a Heatmap showing mRNA fold change in cellular stress markers and, b inflammatory markers in whole pancreatic tissue, relative to control housekeeping gene Ywhaz . c Mean intensity of Grp-78 per islet area ( p = 0.0386 ) and representative image. d Mean intensity of 4-Hne per islet area ( p = 0.0252 ) and representative image. e Percentage of islet area stained by Iba-1 ( p = 0.0024 ) and representative image. f Percentage of islet area stained by MHC II ( p = 0.0034 ) and representative image. All bar graphs are presented as Mean ± SEM. Female animals; a , b n = 7 biologically independent animals. c n = 15 independent islets (all islets in one section) from 3 biologically independent wildtype ( IL-22ra fl/fl ), and 12 independent islets (all islets in one section) from 3 biologically independent IL-22ra1 ΔβKO animals. d n = 7 independent islets (all islets in one section) from 3 biologically independent wildtype ( IL-22ra fl/fl ), and 6 independent islets (all islets in one section) from 3 biologically independent IL-22ra1 ΔβKO animals. e n = 13 independent islets (all islets in one section) from 3 biologically independent wildtype ( IL-22ra fl/fl ), and 10 independent islets (all islets in one section) from 3 biologically independent IL-22ra1 ΔβKO animals. f n = 14 independent islets (all islets in one section) from 3 biologically independent wildtype ( IL-22ra fl/fl ), and 10 independent islets (all islets in one section) from 3 biologically independent IL-22ra1 ΔβKO animals. Two-tailed unpaired Student’s t-test. * p < 0.05, ** p < 0.01; n.s., non-significant. *versus wildtype (IL-22ra1 fl/fl ) littermates. Scale bar: 20 um. Source data are provided as a Source Data file.

Article Snippet: IL-22ra1-FLPe (B6.Cg- Il22ra1 tm1.1Koll /J; Jackson Laboratory Strain #:031003) animals were crossed with either Ins2-cre (B6.Cg-Tg(Ins2-cre)25Mgn/J; Jackson Laboratory Strain #:003573) or Gcg-cre (B6.Cg-Tg(Gcg-cre)1Herr/Mmnc/J; MMRCC, 000358-UNC) animals to generate Ins2 cre x IL-22ra1-FLPe and Gcg cre x IL-22ra1-FLPe colonies respectively.

Techniques: Control, Staining, Two Tailed Test

Journal: Nature Communications

Article Title: Pancreatic beta-cell IL-22 receptor deficiency induces age-dependent dysregulation of insulin biosynthesis and systemic glucose homeostasis

doi: 10.1038/s41467-024-48320-2

Figure Lengend Snippet: a Total insulin secretion (ng/ug protein/min) ( b ) proinsulin secretion (ng/ug protein/min) (90 min; p = 0.0459 ), and c proinsulin: insulin ratio of mouse islets during in-vitro glucose stimulated insulin secretion, following stimulation with 2.8 mM glucose, 20 mM glucose and 20 mM glucose + 100 nM GLP-1. (60 min; p = 0.0182 , 90 min; p = 0.0056 , AUC; p = 0.0076 ). d 2-NBDG uptake in 3T3-L1 adipocytes exposed to 2 ng/mL islet insulin secretion following stimulation with 20 mM glucose + 100 nM GLP-1, p = 0.0054 . All graphs in (a-c) are presented as Mean ± SEM, box plots in ( d ) display the median (central line), 25 th to 75 th percentile (box) and minimum to maximum values (whiskers). Female animals; a – c n = 13 independent samples (10 islets/sample) from 3 biologically independent wildtype ( IL-22ra fl/fl ), and 19 independent samples (10 islets/sample) from 4 biologically independent IL-22ra1 ΔβKO animals. RM two-way ANOVA with the Geisser-Greenhouse correction and Sidak’s multiple comparisons test (line graphs); two-tailed Mann-Whitney Test (bar/box plots). d n = 8 ( IL-22ra fl/fl ) and 9 ( IL-22ra1 ΔβKO ) independent samples. * p < 0.05; n.s., non-significant; ** p < 0.01; n.s., non-significant. *versus wildtype ( IL-22ra fl/fl ) control. Source data are provided as a Source Data file.

Article Snippet: IL-22ra1-FLPe (B6.Cg- Il22ra1 tm1.1Koll /J; Jackson Laboratory Strain #:031003) animals were crossed with either Ins2-cre (B6.Cg-Tg(Ins2-cre)25Mgn/J; Jackson Laboratory Strain #:003573) or Gcg-cre (B6.Cg-Tg(Gcg-cre)1Herr/Mmnc/J; MMRCC, 000358-UNC) animals to generate Ins2 cre x IL-22ra1-FLPe and Gcg cre x IL-22ra1-FLPe colonies respectively.

Techniques: In Vitro, Two Tailed Test, MANN-WHITNEY, Control

Journal: Nature Communications

Article Title: Pancreatic beta-cell IL-22 receptor deficiency induces age-dependent dysregulation of insulin biosynthesis and systemic glucose homeostasis

doi: 10.1038/s41467-024-48320-2

Figure Lengend Snippet: Graphical abstract of findings showing that ablation of pancreatic beta-cell IL-22ra1 signaling leads to increased islet cellular stress and MHC II expression, reduced islet regeneration and insulin biosynthesis, and hypersecretion of proinsulin during glucose stimulation. These factors contribute to the age-related hyperglycaemia observed in IL-22ra1 ΔβKO animals. Figure created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

Article Snippet: IL-22ra1-FLPe (B6.Cg- Il22ra1 tm1.1Koll /J; Jackson Laboratory Strain #:031003) animals were crossed with either Ins2-cre (B6.Cg-Tg(Ins2-cre)25Mgn/J; Jackson Laboratory Strain #:003573) or Gcg-cre (B6.Cg-Tg(Gcg-cre)1Herr/Mmnc/J; MMRCC, 000358-UNC) animals to generate Ins2 cre x IL-22ra1-FLPe and Gcg cre x IL-22ra1-FLPe colonies respectively.

Techniques: Expressing